Fig. 6.

MAIT cell activation after PD-1 blocking. Single cell suspensions were isolated from unaffected colon, colon tumors and peripheral blood, and stimulated with anti-CD3, anti-CD28 and IL-2 (a) or THP-1 cells pre-incubated with E. coli (b) in the presence or absence of blocking antibodies to PD-1. MAIT cell expression of CD25 (a) or co-expression of HLA-DR and CD38 (b) was evaluated by flow cytometry. Symbols represent individual values, and are connected to show corresponding values in the same individuals. *p < 0.05 using Wilcoxon matched-pairs signed rank test. n = 3–6