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. 2021 May 11;70(12):3669–3677. doi: 10.1007/s00262-021-02955-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Antigen-absorption experiments using rhPOMC protein in immunofluorescence and immunoblotting analysis using patients’ serum. a Pre-absorption of the serum with rhPOMC protein diminished the signal in cases #2 and #14, indicating that the autoantibody specifically recognized POMC protein. Scale bar: 50 µm. b The sera in the cases #2 and #14 specifically recognized a 29-kDa protein that corresponded to POMC protein (arrow). The representative results are shown. No sera from other cases nor healthy subjects exhibited anti-POMC antibody