Fig. 2.

Mitazalimab induces the release of proinflammatory cytokines and chemokines in the blood—MB49 tumor-bearing hCD40tg mice were administered a single dose of 10, 30, 100 or 300 µg mitazalimab i.p. on day 7 post-inoculation, and 24 h after dosing, blood was collected via vena saphena for MSD analysis. The V-PLEX Mouse Cytokine 19-Plex Kit was used to measure CXCL1 (KC/GRO), IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-15, IL-17A/F, IL-27p28/IL-30, IL-33, IP-10, MCP-1, MIP-1α, MIP-2 and TNF-α. Only the cytokines/chemokines that showed a consistent change across two experiments are shown. n = 2–5 per group. Statistical significance was analyzed by Mann–Whitney U test. The lower limit of detection (LLOD) is defined by a horizontal dotted line. All error bars indicate ± SEM