TABLE 3.
DNA restriction enzyme endonucleolytic cleavage sites and fragment sizes for three different RT-PCR products derived from CDV NP RNA
| Restriction enzyme | Sequence around cleavage site (5′-3′)a | PP-I
|
PP-II
|
PP-III
|
|||
|---|---|---|---|---|---|---|---|
| Site(s) | Fragment size (bp) | Site | Fragment size (bp) | Site(s) | Fragment size (bp) | ||
| AluI | AG↓CT | 129, 138 | 9, 129, 149 | 244 | 16, 244 | 187, 196, 474, 526, 545, 567, 572, 621 | 5, 9, 19, 22, 49, 52, 187, 278, 279 |
| BsiMI | T↓CCGGA | 207 | 80, 207 | 34 | 34, 226 | 264 | 264, 636 |
a
The arrows indicate the cleavage sites.