TABLE 4.

Detection of CDV NP nucleic acid by RT-PCR and virus-specific neutralizing antibody titers in dogs with distemper

Dog no.	Clinical form of distempera	RT-PCR result with PP-I			Neutralizing antibody titer
		Serum	CSF	Whole blood	Serum	CSF
13	NI	+	NAb	NA	1:80	NA
14	C	+	NA	NA	1:280	NA
15	C	+	−	+	1:67	NA
16	N	+	NA	NA	NA	NA
17	S	+	NA	NA	NA	NA
18	S	+	NA	NA	1:540	NA
19	S	+	+	+	1:113	NA
20	C	+	NA	NA	<1:80	NA
21	C	+	+	+	1:56	<1:10
22	S	+	NA	NA	<1:40	NA
23	C	+	+	+	1:80	1:20
24	C	+	NA	NA	1:320	NA
25	S	+	+	+	NA	<1:10
26	S	+	+	+	1:190	<1:10
27	C	+	+	+	<1:10	<1:10
28	C	−	+	−	1:10	NA
29	C	+	+	+	NA	<1:10
30	C	+	+	+	<1:10	NA
31	S	+	+	+	1:20	<1:10
32	S	+	+	+	<1:10	<1:10
33	C	+	−	+	1:23	<1:10
34	S	±	+	+	NA	NA
35	S	+	NA	NA	NA	NA
36	S	−	NA	NA	1:134	NA
37	S	−c	NA	NA	1:40	NA
38	N	±	±	−	1:80	NA
39	S	±	NA	NA	<1:10	NA
40	N	+	+	+	1:28	1:40
41	N	−	NA	NA	1:190	NA

^aNI, no information available; C, catarrhalic; N, nervous; S, systemic. 

^bNA, not available. 

^cNegative on the ethidium bromide-stained agarose gel but positive by Southern blotting.