Fig. 3.

Longitudinal flow cytometric phenotyping and plasma cytokine level a Phenotyping of peripheral blood leukocytes was done before, during therapy and at the endpoint. Therefore, blood was collected via the retrobulbar venous plexus and stained with antibodies. Given are the percentage numbers of positive cells ± SD resulting from 20,000 events measured on a flow cytometer. b Plasma cytokine levels from mice with immunotherapy and controls (upper graph). Differences between tumor-free and tumor-bearing mice (lower graphs). Plasma samples were collected at the experimental endpoint and cytokine levels determined as described in material and methods. Given are the mean cytokine level ± SD