Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 18;70(12):3405–3419. doi: 10.1007/s00262-021-02933-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Immunofluorescence a, c Residual Mlh1^−/− GIT were resected after therapeutic vaccination and cryostat sections of 4 µm prepared. Tumor microenvironment was studied upon staining with specific mAbs, followed by nuclear staining with DAPI. Pictures were taken on a laser scanning microscope (Zeiss) using 20× objectives. b, d Quantification of infiltrating immune cells. At least three pictures were

taken from each slide and numbers of infiltrating cells counted. Data are given as infiltrates/HPF. Mean + SD, n ≥ 3 samples/group; *p < 0.05, **p<0.01; ***p<0.001 one-way ANOVA (Bonferroni’s multiple comparison test)

Fig. 6 — Immunofluorescence a, c Residual Mlh1^−/− GIT were resected after therapeutic vaccination and cryostat sections of 4 µm prepared. Tumor microenvironment was studied upon staining with specific mAbs, followed by nuclear staining with DAPI. Pictures were taken on a laser scanning microscope (Zeiss) using 20× objectives. b, d Quantification of infiltrating immune cells. At least three pictures were

taken from each slide and numbers of infiltrating cells counted. Data are given as infiltrates/HPF. Mean + SD, n ≥ 3 samples/group; *p < 0.05, **p<0.01; ***p<0.001 one-way ANOVA (Bonferroni’s multiple comparison test)