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. 2021 Nov 5;11:21741. doi: 10.1038/s41598-021-01206-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representation of the A. baumannii AB5075-UW chromosome showing location of wzi gene (blue) in relation to the K locus and attTn7 site (red). Institut Pasteur MLST alleles and the intrinsic oxaAB gene are also shown. (B) Genetic location of T26 insertion sites in A. baumannii AB5075-UW transposon library mutants. Genes, locus tags, and sequence lengths are indicated below the gene. Triangles represent insertion sites at listed base positions in gene sequence. Insertions in the forward strand shown above and insertions in reverse orientation shown below. The intergenic region that includes a predicted promoter sequence for wzi (blue) is shown upstream (yellow). (C) Composition of the chimeric gene cassette used to complement wzi::T26 mutant strains. Size of amplicons are indicated along with the positions of oligonucleotide primers used for amplification. Oligonucleotide sequences are listed in Supplementary Table S5.