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. 2021 Nov 5;12:6263. doi: 10.1038/s41467-021-26554-8

Fig. 4. Diversification of the NLR gene cluster across 30 diverse soybean genomes.

Fig. 4

The phylogenetic tree on the left side was built using SNP data with the Rps11 region. Orange color highlights the four major haplotype groups. Each black box represents an NLR gene. Gray shades represent syntenic blocks among genomes. Light-blue highlight inversion events. Green highlight tandem duplication events. Light-red highlight potential unequal recombination events. Box 1 and Box 2 exemplify a deletion and a duplication event induced by unequal recombination, respectively. The name of each genome was labeled at the right side of each cluster and the numbers in parentheses are the total copy number of NLR genes in each genome at the Rps11 corresponding region. Box 3 shows the sequence comparison of the promoter regions of Rps11 and that of W05.r5 and W05.r6. Light-blue shades represent syntenic blocks. Box 4 shows the comparison of the expression levels of NLR genes with TSR (TSR+, n = 187 NLR genes from 30 soybean accessions) and without TSR (TSR−, n = 129 NLR genes from 30 soybean accessions) in the Rps11 region and “rps11” regions across all the genomes. Horizontal lines indicate the medians and the boxes represent the interquartile range (IQR). The whiskers represent the range of 1.5 times IQR and dots beyond the whiskers are outlier values. The statistical significance was determined by a two-sided t-test and P-value is shown above the plot. Source data underlying Box 4 are provided as a Source Data file.