| YPD media | ||
|---|---|---|
| Reagent | Final concentration | Amount |
| Yeast Extract | 1% | 10 g |
| Peptone | 2% | 20 g |
| Dextrose | 2% | 20 g |
| dH2O | n/a | 1,000 mL |
| Total | n/a | 1,000 mL |
To prepare the media, yeast extract and peptone (YEP) is dissolved in 900 mL of dH2O and autoclaved. To avoid the Maillard reaction (a chemical reaction between amino acid and reducing sugar that will change the medium into a brownish color, dropping the actual sugar concentration (Wang and Hsiao, 1995)), it is recommended to dissolve the 20 g dextrose separately in 100 mL dH2O and sterile filter (0.22 μm). 100 mL of the sterile 20% dextrose solution is finally added to 900 mL YEP under sterile conditions, obtaining 1 L of YPD media.