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. 2021 Oct 8;297(5):101287. doi: 10.1016/j.jbc.2021.101287

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Characterization of cYY binding in CYP121A1 measured by the FG loop.¹⁹F-NMR spectra of 1:0 versus 1:2 ratios (A) and 1:2 versus 1:20 ratios (C) of CYP:cYY. UV-visible absorbance difference spectra of 1:2 ratio (B) and 1:2 versus 1:20 ratios (D) of CYP:cYY. E, ¹⁹F-NMR spectra of ligand-free (black), cYY-bound (orange), and dialyzed (green) CYP121A1. F, UV-visible absorbance difference spectra of high-spin shifted (orange) and subsequently dialyzed CYP121A1 (green). UV-absorption spectra of dilutions of CYP121A1 in the presence of equimolar cYY, monitoring changes in the Soret band (G) and the Q-band spectra (H).