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. Author manuscript; available in PMC: 2021 Nov 6.
Published in final edited form as: Nat Med. 2021 Jul 8;27(8):1357–1361. doi: 10.1038/s41591-021-01403-9

Extended Data Figure 3. Analysis of phage resistance in vitro.

Extended Data Figure 3.

a, 1 ml of 2 × 108 CFU GD82 was mixed with 2 × 109 PFU of each phage used in the cocktail or with all three phages, and incubated at 37° C with shaking. After 48 hours, 0.1 ml of the mix was spread on solid media and incubated for 5 days at 37° C. b, Four surviving colonies of each phage challenge were grown and retested for phage sensitivity by spotting 10-fold serial dilutions of phages as indicated. GD82-M_RM2 and GD82-Z_RM2 could not be propagated and were not tested further. BPsΔ33HTH_HRM10 RMs were not tested.