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. 2000 Jun;20(11):3860–3869. doi: 10.1128/mcb.20.11.3860-3869.2000

FIG. 2.

FIG. 2

Efficient binding of DDP1 to the dodeca-satellite C strand depends on the extent of secondary structure of the DNA substrate. (A) The binding of DDP1 to oligo 9R (panel 9R) and oligo 9Rc (panel 9Rc) is shown as a function of increasing excess quantities (weight to weight) of single-stranded (ss) E. coli DNA: 0 (lanes 0), 25 (lanes 1), 250 (lanes 2), and 1,000 (lanes 3). (B) The binding of DDP1 to oligo 9R is shown as a function of increasing amounts of oligo 9R (panel 9R) and oligo 9Rc (panel 9Rc). Excess quantities (weight to weight) of competitor used were as follows: panel 9R, 5 (lane 1), 50 (lane 2), and 500 (lane 3); and panel 9Rc, 50 (lane 1), 500 (lane 2), and 2,500 (lane 3). Lane 0 shows the binding obtained in the absence of any added competitor. Quantitative analysis of the results are shown to the right of each panel for oligo 9R and oligo 9Rc. See Table 1 for a description of the DNA fragments used in these experiments.