| Primer | Sequence (5’ – 3′) | Annealing temperature (oC) |
|---|---|---|
| T7 forward | TAATACGACTCACTATAGG | 55 |
| T7 terminator | GCTAGTTATTGCTCAGCGG | |
| 5TpET28 | GGAATTGTGAGCGGATAAC | 55 |
| M13F | GTAAAACGACGGCCAG |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.