PCR cycling conditions
Step	Temperature (°C)	Time	Cycles
Initial denaturation	95	3 min	1
Denaturation	95	30 s	20
Annealing	55	30 s
Extension	72	-- min∗
Final extension	72	5 min	1
Hold	10	∞

∗Extension time varies depending on the indicated gene:

polA gene (Taq DNA polymerase): 3 min

pol gene (M-MLV Reverse transcriptase): 3 min

cas12a gene (LbCas12a): 5 min 30 s

p1 gene (TEV protease): 2 min 30 s