| Reagent | Initial concentration | Final concentration | Volume (μL) per reaction |
|---|---|---|---|
| RPB4X | 4X | 1X | 5 |
| M-MLV RT | 20X | 1X | 1 |
| Taq polymerase | 20X | 1X | 1 |
| Reverse primer | 5 μM | 0.2 μM | 0.8 |
| Forward primer | 5 μM | 0.2 μM | 0.8 |
| Nuclease-free water | - | - | 9.4 |
| Sample/controls | - | - | 2 |
| Final volume | 20 |
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