| PCR cycling conditions | |||
|---|---|---|---|
| Step | Temperature (°C) | Time | Cycles |
| Reverse transcription | 50 | 20 min | 1 |
| Initial denaturation | 95 | 5 min | 1 |
| Denaturation | 95 | 5 s | 45 |
| Annealing/Extension | 55 | 30 s | |
| Hold | 10 | ∞ | |
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