| Reagent | Final concentration per reaction |
|---|---|
| LbCas12a | 0.1μM |
| Re-folded crRNA | 0.15 μM |
| Dual-labeled single-stranded DNA (ssDNA) reporter probe | 3 μM |
| 1X CrB1∗ | - |
| Final volume | 10 μL |
∗1X CRISPR/Cas buffer 1, see Data S1.
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