| Pipetting Order |
Reagent | Initial concentration | Final concentration | Final volume (μL) per assay | Notes |
|---|---|---|---|---|---|
| 1 | 1X CrB2 (17.65 mM MgCl2) | 1X | 1X | 85 | Mix separately |
| 2 | RT-PCR product or 1 μM synthetic template | - | - | 5 | |
| 3 | CRISPR-Cas12a complex | 10X | 1X | 10 | |
| Final volume | - | - | 100 |
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