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1H NMR (400MHz) spectra of media collected from a sgGlo1 pLHCX cells (re-expressed GLO1), b sgGlo1 (GLO1 deleted cells), and c parental 3353T3 cells. Each cell line was incubated with 5 mM methylglyoxal (MG) in DMEM basal media for 4h, 37°C, and pH 7 and mixed with 10-mM Yb3 prior to collection of the NMR spectra. The highlighted methyl and methine resonances are assigned to l-(blue) and d-(red) lactate. Western blots showing GLO1 expression in 3353T3 cells
