Table 2.

PCR quantification of Tn5253 circular forms and reconstituted attB integration sites in Tn5253-carrying transconjugants

Strain	Genetic background	Circular Forms	Reconstituted attB Site	Conjugation Frequency Mean (Range)a
FR39	S. pneumoniae A66	2.0 × 10−5 ± 1.9 × 10−5	<6.9 × 10−6 ± 7.1 × 10−8	4.4 × 10−7 (3.2 × 10−7 - 5.8 × 10−7)
FR54	S. pneumoniae TIGR4	<3.6 × 10−5 ± 2.6 × 10−6	<3.5 × 10−4 ± 2.6 × 10−5	<9.9 × 10−8 (<9.6 × 10−8 - <1.3 × 10−7)
FR56	S. pneumoniae SP18-BS74	1.2 × 10−2 ± 7.5 × 10−5	1.9 × 10−3 ± 1.0 × 10−4	6.1 × 10−6 (3.5 × 10−6 - 9.5 × 10−6)
FR67	S. agalactiae H36B	2.9 × 10−5 ± 8.7 × 10−6	3.0 × 10−4 ± 1.0 × 10−4	1.1 × 10−6 (3.2 × 10−7 - 2.1 × 10−6)
FR68	S. pyogenes SF370	3.0 × 10−5 ± 1.0 × 10−5	3.7 × 10−5 ± 6.2 × 10−6	9.5 × 10−4 (3.3 × 10−5 - 2.6 × 10−3)
FR43	S. gordonii V288	6.5 × 10−5 ± 4.4 × 10−5	7.9 × 10−5 ± 1.2 × 10−5	8.3 × 10−7 (1.2 × 10−7 - 2.0 × 10−6)
FR49	E. faecalis OG1SS	1.4 × 10−4 ± 9.2 × 10−5	6.8 × 10−3 ± 1.0 × 10−4	<1.8 × 10−8 (<1.0 × 10−9 - <3.9 × 10−8)
FR50	E. faecalis JH2-2	<2.7 × 10−7 ± 7.5 × 10−8	1.7 × 10−2 ± 1.3 × 10−3	<2.7 × 10−8 (<9.9 × 10−9 - <5.0 × 10−8)

^aFrequency refers to mating experiments where S. pneumoniae FP10 or FP11was the conjugation recipient [29]; conjugation frequency is expressed as

CFU of transconjugants per CFU of donors; each result is the mean of at least three mating experiments