TABLE 3.
Direct and indirect agglutination of the MAba
| Microorganism(s) cultured | No. positive/no. tested
|
||||
|---|---|---|---|---|---|
| Blood culture
|
BHIB
|
Primary plate and direct agglutination | |||
| Direct agglutination | Latex agglutinationb | Direct agglutination | Latex agglutination | ||
| Burkholderia pseudomalleic | 24/51 | 51/51 | 48/51 | 51/51 | 51/51 |
| Acinetobacter spp. | 0/9 | 3/9 | 1/9 | 1/9 | 0/9 |
| Aeromonas spp. | 1/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| Burkholderia cepacia | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| Citrobacter diversus | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 |
| Enterobacter spp. | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 |
| Escherichia coli | 1/36 | 3/36 | 1/36 | 0/36 | 0/36 |
| Haemophilus influenzae | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Klebsiella ozaenae | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Klebsiella pneumoniae | 0/18 | 4/18 | 0/18 | 0/18 | 0/18 |
| Plesiomonas shigelloides | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Pseudomonas spp. | 1/8 | 2/8 | 0/8 | 1/8 | 0/8 |
| Pseudomonas aeruginosa | 1/12 | 4/12 | 0/12 | 0/12 | 0/12 |
| Proteus mirabilis | 0/3 | 0/3 | 0/3 | 2/3 | 0/3 |
| Salmonella spp. | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Salmonella paratyphi A | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Group B Salmonella | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 |
| Group D Salmonella | 1/8 | 0/8 | 0/8 | 0/8 | 0/8 |
| Salmonella typhi | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Vibrio parahaemolyticus | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| E. coli and K. pneumoniae | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
Direct agglutination against B. pseudomallei was measured. In indirect agglutination, the MAb adsorbed to latex particles with bacteria in blood culture broth, in subcultured BHIB, or on a primary plate is also shown.
The false positive results in some specimens could be eradicated by centrifugation at 1,000 × g for 5 min before the agglutination was performed.
The sensitivity (%) and specificity (%) of agglutination of the MAb with B. pseudomallei are as follows. Values listed after culture method and assay indicate sensitivity, followed in parentheses by specificity, for 51 specimens tested. Blood culture (direct agglutination), 47.06 (95.61); blood culture (latex agglutination), 100 (85.96); BHIB (direct agglutination), 94.12 (98.25); BHIB (latex agglutination), 100 (96.49); primary plate (direct agglutination), 100 (100).