Table 1.

performance of the PANDAA assay with Sanger sequencing as the reference method

Mutations	True positive	True negative	False positive	False negative	Kappa value (95% CI)
Overall DRMs	17	101	2	0	0.93(0.78-0.98)
NRTI DRMS	3	117	0	0	1.00(0.54-1.00)
K65R	0	120	0	0	#
M184V	3	117	0	0	1.00 (0.54-1.00)
NNRTI DRMS	16	102	2	0	0.93(0.77-0.98)
K103N	15	103	2	0	0.93(0.76-0.98)
Y181C	0	119	1	0	#
G190A	3	117	0	0	1.00(0.54-1.00)

# = not computed; CI= confidence interval; DRMs= drug resistance mutations; NRTI DRMs= frequency of nucleotide reverse transcriptase inhibitor mutations detected either individually or together (K65R and/or M184V); NNRTI DRMs= frequency of non-nucleoside reverse transcriptase inhibitor mutations detected either individually or together (K103N and/or Y181C and/or G190A).