Fig. 2.

mask knockdown increases the sedimentation of MTs in fly larval muscles. (A) Western analysis (representative of three experiments) of β-tubulin protein in total lysate or ultracentrifugal fractions (supernatant and pellet) in larval muscle lysates from control or mask knockdown (24B-Gal4-driven mask RNAi) animals. Lysates were treated with either 100 µm or 100 nm Taxol before ultracentrifugation. Anti-β-tubulin and anti-α-actin blots were performed for the western analysis. (B) Quantification of the total tubulin levels in the tissue lysates. The levels of tubulin were normalized to the levels of total α-actin in the same sample. (C) Quantification of the tubulin levels in the supernatant after centrifugation. The levels of tubulin were normalized to the levels of total actin in the same sample. (D) Quantification of the percentage of tubulin proteins that remained in the supernatant after centrifugation to total tubulin proteins present in the tissue lysate. (E) Quantification of the relative levels of tubulin fractionated in the pellet fraction in the lysate treated with 100 nm Taxol. The levels of tubulin in pellet were normalized to the levels of total tubulin in the same sample. The relative tubulin levels in the mask knockdown samples were then normalized to the relative tubulin levels in the control samples.