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. 2021 Oct 19;148(20):dev199591. doi: 10.1242/dev.199591

Fig. 6.

Fig. 6.

Removal of p53 in the Rnu11 cKO inhibits cell death and delays DNA damage. (A) IHC for γH2AX (green), CC3 (magenta) and TUNEL (yellow) on sagittal sections of control (left), Rnu11 cKO (middle) and dKO (right) pallium across E12.5 (A′), E13.5 (A″) and E14.5 (A‴). Nuclei are marked by DAPI (blue). Scale bars: 30 µm. (B) Quantification of cell death via TUNEL (top) and CC3 (bottom) signal in control, Rnu11 cKO and dKO pallium across E12.5, E13.5 and E14.5. (C) Quantification of DNA damage via γH2AX signal in control, Rnu11 cKO and dKO pallium across E12.5, E13.5 and E14.5. E12.5 control n=4, Rnu11 cKO n=4, dKO n=4. E13.5 control n=5, Rnu11 cKO n=5, dKO n=5. E14.5 control n=4, Rnu11 cKO n=4, dKO n=4. Data are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001 (one-way ANOVA, followed by post-hoc Tukey test). n.s., not significant.