Fig. 4.

Blocking phosphorylation promotes endocytosis of RLP44-GFP. (A) Inducible expression of amiRNAs targeting the clathrin adapter complex TPLATE leads to reduced formation of endosomes and decreased uptake of FM4-64 into BFA bodies compared to mock control, suggesting efficient blockage of clathrin-mediated endocytosis. Knockdown of TPLATE in RLP44-GFP Pdead leads to enhanced plasma membrane localization. Scale bars: 10 µm. (B) Quantification of mean plasma membrane to intracellular fluorescence ratio in the indicated genotypes with and without amiRNA-mediated knock down of TPLATE. Boxes indicate range from 25th to 75th percentile, horizontal line indicates the median, whiskers indicate data points within 1.5 time the interquartile range. Markers above whiskers indicate outliers; n (cells) from 15–18 independent roots each are indicated. Different letters indicate statistically significant differences (P<0.05) from other letter groups according to Dunn's post hoc test after one-way Kruskal–Wallis test.