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Effect of anti- TLR 4 antibodies on IL-6 production in LPS-induced U937 cells cultured in absence of FBS. Human U937 monocytes were treated with LPS and/or anti-TLR 4 antibodies. IL-6 levels in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA) after 24 h of treatment. *P < 0.0001 vs control; †P < 0.05 vs LPS 100 pg/mL. ‡P < 0.0005 vs LPS 1 μg/mL.
Abbreviations: IL, interleukin; LPS, lipopolysaccharide; A-TLR, anti-Toll-like receptor.
