TABLE 3.
Amplification of cultivable PROS isolates by species-specific PCRa
| Treponema tested | Results of species-specific PCR withb:
|
||
|---|---|---|---|
| T. medium (192) | T. pallidum (198) | T. vincentii (193) | |
| PROS isolates | |||
| OHSU 242-9 | Not amplified | Not amplified | Amplified |
| OHSU 242-10 | Not amplified | Not amplified | Amplified |
| OMZ-802 | Not amplified | Not amplified | Amplified |
| OMZ-804 | Not amplified | Not amplified | Not amplified |
| OMZ-805 | Amplified | Not amplified | Not amplified |
| Control treponema | |||
| T. medium | Amplified | Not amplified | Not amplified |
| T. pallidum | Not amplified | Amplified | Not amplified |
| T. vincentii | Not amplified | Not amplified | Amplified |
a
Each species-specific PCR produced a characteristic and reproducible product only with the appropriate template (16). Each experiment included both positive and negative controls, and each experimental PCR product was identical in size to control amplicons.
b
The product size in base pairs is in parentheses.