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. 2021 Nov 6;7:337. doi: 10.1038/s41420-021-00729-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative confocal microscopy micrographs of AML-12 cells after co-culture with purified PKH67-EVs. b HMGB1-GFP EVs uptake occurred in vitro and in vivo. c The co-localization of internalized EVs and AML-12 endosomes (EEA1, Rab5, Rab7) or Lamp1. d The expression of transferrin receptor in EVs, the co-localization of internalized EVs with AML-12 transferrin. e The LPS-EVs absorbed by AML-12 cells. f Cytoplasmic HMGB1 in AML-12 cells. (**) P < 0.01 versus control group. (^##) P < 0.01 versus EVs group.