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. 2021 Nov 6;12(11):1056. doi: 10.1038/s41419-021-04117-5

Fig. 5. EPC-derived EVs carrying TUG1 stimulates macrophages M2 polarization by upregulating SIRT1 expression through inhibition of miR-9-5p.

Fig. 5

A The expression of miR-9-5p and SIRT1 in macrophages transfected with pcDNA-TUG1 or miR-9-5p mimic or their NCs determined by RT-qPCR. B Western blot analysis for determination of protein expression of SIRT1 in macrophages transfected with pcDNA-TUG1 or miR-9-5p mimic or their NCs. C RT-qPCR for measurement of mRNA expression of iNOS and TNF-α in macrophages transfected with pcDNA-TUG1 or miR-9-5p mimic or their NCs D RT-qPCR for measurement of the mRNA expression of IL-10 and Arg-1 in macrophages transfected with pcDNA-TUG1 or miR-9-5p mimic or their NCs. E IL-10 and TNF-α contents in the medium detected by ELISA. F The protein expression of iNOS and Arg-1 in macrophages transfected with pcDNA-TUG1 or miR-9-5p mimic or their NCs measured by western blot analysis. G RT-qPCR for determination of mRNA expressions of miR-9-5p and SIRT1 in macrophages cocultured with EPCs derived EVs measured by. H The protein expression of SIRT1 in macrophages cocultured with EPCs derived EVs examined by western blot analysis. I Determination of the mRNA expression of iNOS, TNF-α, IL-10, and Arg-1 in macrophages cocultured with EPCs derived EVs by RT-qPCR. J The levels of IL-10 and iNOS in the medium assessed by ELISA. K Measurement of the protein expression of iNOS and Arg-1 in macrophages cocultured with EPCs derived EVs by western blot analysis. Measurement data were expressed as mean ± standard deviation. One-way ANOVA was adopted for comparison between multiple groups followed by Tukey’s post hoc test. Cell experiments were conducted three times independently. *p < 0.05 vs. LPS group; #p < 0.05 vs. LPS + EVs + mimc-NC or inhibitor-NC or LPS + TUG1 + mimic NC group.