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. 2021 Nov 6;22:368. doi: 10.1186/s12882-021-02580-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — NNT-AS1 targets miR-214-5p. A Potential binding elements of miR-214-5p in NNT-AS1 identified through LncBASE database, B relative luciferase assay confirming reduced miR-214-5p expression in HGMC lines inoculated with wild-type (WT) and mutant NNT-AS1, C RNA pull-down experiment using biotin-labeled NNT-AS1 probe showing more pulled down miR-214-5p (P < 0.01) as compared to oligo probe, D RNA immunoprecipitation demonstrating more enrichment of NNT-AS1 and miR-214-5p by Ago2 in comparison to IgG group, E qRT-PCR analysis showing elevated miR-214-5p expression in the HGMC groups transfected with si-NNT-AS1#1 and si-NNT-AS1#2, F the expression level of miR-214-5p in blood samples of 22 patients with DN and 20 normal persons was detected by qRT-PCR, G Spearman correlation coefficient showing significant negative correlation between the expression of NNT-AS1 and miR-214-5p