TABLE 2.
Restriction fragments of polymorphic mycobacterial genes as determined by direct sequencing and FCE
| Species | End fragment size (bp)a
|
|||
|---|---|---|---|---|
| 65-kDa HSP gene
|
16S rRNA gene
|
|||
| BstEII | HaeIII | CfoI | HaeIII | |
| M. tuberculosis | 116 (113), 231 (230) | 127 (126), 152 (150) | 149 (148), 160 (160) | 31 (33), 187 (186) |
| M. bovis | 116 (113), 231 (230) | 127 (126), 152 (150) | 149 (148), 160 (160) | 31 (33), 187 (186) |
| M. bovis BCG | 116 (113), 231 (230) | 127 (126), 152 (150) | 149 (148), 160 (160) | 31 (33), 187 (186) |
| M. africanum | 116 (113), 231 (230) | 127 (126), 152 (150) | 149 (148), 160 (160) | 31 (33), 187 (186) |
| M. microti | 210 (208), 231 (230) | 40 (40), 127 (124) | 138 (139), 146 (147) | 31 (32), 178 (177) |
| M. avium | 210 (208), 231 (230) | 40 (40), 127 (126) | 149 (148), 326 (325) | 31 (32), 178 (179) |
| M. intracellulare | 116 (112), 231 (231) | 40 (41), 127 (126) | 139 (136), 149 (148) | 27 (28), 31 (32) |
| M. simiae | 210 (231), 231 (228) | 126 (123), 185 (182) | 62 (60), 138 (136) | 26 (28), 31 (32) |
| M. gordonae | 116 (113), 231 (229) | 40 (38), 161 (158) | NF | 28 (28), 31 (32) |
| M. kansasii | 210 (207), 231 (229) | 127 (123), 185 (181) | 139 (136), 149 (148) | 31 (32), 178 (177) |
| M. fortuitum | 116 (113), 231 (231) | 41 (43), 145 (142) | 62 (59), 134 (130) | 31 (32), 178 (165) |
| M. peregrinum | 210 (208), 231 (233) | 98 (102), 139 (136) | 62 (59), 134 (130) | 31 (30), 178 (165) |
| M. chelonae | 131 (128), 310 (310) | 40 (44), 197 (195) | 137 (137), 325 (325) | 31 (30), 184 (182) |
| M. abscessus | 210 (207), 231 (232) | 40 (40), 145 (142) | 137 (136), 326 (326) | 31 (31), 185 (183) |
| M. celatum | 210 (208), 231 (231) | 40 (39), 127 (123) | 138 (139), 324 (328) | 31 (30), 176 (180) |
| M. marinum | 210 (208), 231 (231) | 40 (40), 106 (103) | 149 (148), 326 (325) | 31 (32), 178 (178) |
| M. asiaticum | 210 (208), 231 (231) | 40 (40), 106 (103) | 139 (139), 149 (148) | 31 (30), 178 (179) |
| M. mucogenicum | 116 (113), 310 (307) | 40 (36), 145 (141) | 31 (30), 136 (133) | 101 (97), 178 (177) |
| M. malmoense | 116 (119), 231 (229) | 40 (42), 106 (105) | 139 (138), 149 (148) | 31 (30), 178 (177) |
| M. gastri | 131 (127), 231 (229) | 40 (38), 127 (127) | 139 (136), 149 (148) | 31 (31), 178 (178) |
| M. scrofulaceum | 210 (206), 231 (230) | 40 (40), 127 (123) | 138 (136), 146 (144) | 26 (29), 31 (33) |
| M. szulgai | NF | 40 (40), 127 (123) | 149 (150), 160 (168) | 27 (28), 31 (32) |
| M. xenopi | 116 (113), 231 (229) | 40 (40), 161 (158) | 133 (135), 139 (143) | 26 (29), 96 (99) |
a
FCE values are in parentheses. NF, no restriction fragments.