TABLE 3.
Analytical sensitivity of MPB64-ICAa
No. of mycobacteria (log CFU/ml) |
MPB64-ICA result |
---|---|
7 | +++ |
6 | ++ |
5 | + |
4 | ± |
3 | − |
2 | − |
Tenfold dilutions of M. bovis BCG grown in Middlebrook 7H9 liquid medium were made in 10 mM phosphate buffer–0.1% Tween 80 and vortexed, and then 100 μl of each dilution was assayed by MPB64-ICA.