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. 2021 May 30;11(6):520–531. doi: 10.1016/j.jtcme.2021.05.004

Fig. 6.

Fig. 6

Histopathology. The hematoxylin and eosin-stained gastrocnemius muscle sections of (a) Healthy control, (b) Disease control, (c) 0.2 g/kg EEMI and (d) 0.4 g/kg EEMI treated animals were observed under phase-contrast microscope under magnification of 10 and 40× (inset). Black arrow: Position of nucleus; Yellow arrow: Myofibers attachment; The nuclei were visible, peripherally located in healthy (a) and EEMI (0.2 and 0.4 g/kg) treated (c and d); whereas the nuclei were moved to centre and some of the nuclei disappeared due to necrosis/injury of the tissue and damage of myofibers were also clearly visible (b).