FIGURE 3.

The effects of EV‐miRNA‐22 on the levels of GSDMD and inflammatory factors. (A) IF staining of GSDMD. LPS/NG promoted the expression of GSDMD, while EV and EV‐miRNA‐22 could inhibit the expression of GSDMD, with significantly decreased fluorescence intensity. (B–D) Detection of inflammatory factor expression. The expression of inflammatory factors (IL‐1β, IL‐18 and TNF‐α) was significantly up‐regulated in pyroptosis, which was higher than that of Control group. EV and EV‐miRNA‐22 could inhibit the release of inflammatory factors. In particular, the level of inflammatory factors was lower in EV‐miRNA‐22 group than that in EV group. Comparison with Control group, *p < 0.05; comparison with LPS + NG group, ^# p < 0.05. (E and F) Protein detection revealed that EV‐miRNA‐22 could inhibit the expression of GSDMD and p30‐GSDMD. Comparison with Control group, *p < 0.05; comparison with LPS + NG group, ^# p < 0.05