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. 2021 Oct 5;25(21):10152–10163. doi: 10.1111/jcmm.16952

TABLE 1.

Gene‐specific primers used for RT‐qPCR

Gene Primer sequence
Bax

Forward 5′‐GACGCATCCACCAAGAAGCTGAG‐3′

Reverse 5′‐GCTGCCACACGGAAGAAGACC‐3′

Bcl‐2

Forward 5′‐AGCATGCGACCTCTGTTTGA‐3′

Reverse 5′‐TCACTTGTGGCCCAGGTATG‐3′

DRP‐1

Forward 5′‐ACAACAGGAGAAGAAAATGGAGT‐3′

Reverse 5′‐CGTTGGGCGAGAAAACCTTG‐3′

Fis‐1

Forward 5′‐GAATACGCCTGGTGCCTGGTTC‐3′

Reverse 5′‐GAAGACATAATCCCGCTGCTCCTC‐3′

OPA‐1

Forward 5′‐ATTTCGCTCCTGACCTGGAC‐3′

Reverse 5′‐GGTGTACCCGCAGTGAAGAA‐3′

MFN‐1

Forward 5′‐CGTGGCAGCAGCAGAGAAGAG‐3′

Reverse 5′‐CCTCCTCCGTGACCTCCTTGATC‐3′

MFN‐2

Forward 5′‐AGAGGCGATTTGAGGAGTGC‐3′

Reverse 5′‐CGCTCTTCCCGCATTTCAAG‐3′

PGC‐1α

Forward 5′‐CCTCACACCAAACCCACAGAGAAC‐3′

Reverse 5′‐TTGCGACTGCGGTTGTGTATGG‐3′

NRF‐1

Forward 5′‐TCTGCTGTGGCTGATGGAGAGG‐3′

Reverse 5′‐GATGCTTGCGTCGTCTGGATGG‐3′

TFAM

Forward 5′‐GCTAAACACCCAGATGCAAAA‐3′

Reverse 5′‐CGAGGTCTTTTTGGTTTTCC‐3′

β‐actin

Forward 5′‐TGTCACCAACTGGGACGATA‐3′

Reverse 5′‐GGGGTGTTGAAGGTCTCAAA‐3′