TABLE 1.
Gene | Primer sequence |
---|---|
Bax |
Forward 5′‐GACGCATCCACCAAGAAGCTGAG‐3′ Reverse 5′‐GCTGCCACACGGAAGAAGACC‐3′ |
Bcl‐2 |
Forward 5′‐AGCATGCGACCTCTGTTTGA‐3′ Reverse 5′‐TCACTTGTGGCCCAGGTATG‐3′ |
DRP‐1 |
Forward 5′‐ACAACAGGAGAAGAAAATGGAGT‐3′ Reverse 5′‐CGTTGGGCGAGAAAACCTTG‐3′ |
Fis‐1 |
Forward 5′‐GAATACGCCTGGTGCCTGGTTC‐3′ Reverse 5′‐GAAGACATAATCCCGCTGCTCCTC‐3′ |
OPA‐1 |
Forward 5′‐ATTTCGCTCCTGACCTGGAC‐3′ Reverse 5′‐GGTGTACCCGCAGTGAAGAA‐3′ |
MFN‐1 |
Forward 5′‐CGTGGCAGCAGCAGAGAAGAG‐3′ Reverse 5′‐CCTCCTCCGTGACCTCCTTGATC‐3′ |
MFN‐2 |
Forward 5′‐AGAGGCGATTTGAGGAGTGC‐3′ Reverse 5′‐CGCTCTTCCCGCATTTCAAG‐3′ |
PGC‐1α |
Forward 5′‐CCTCACACCAAACCCACAGAGAAC‐3′ Reverse 5′‐TTGCGACTGCGGTTGTGTATGG‐3′ |
NRF‐1 |
Forward 5′‐TCTGCTGTGGCTGATGGAGAGG‐3′ Reverse 5′‐GATGCTTGCGTCGTCTGGATGG‐3′ |
TFAM |
Forward 5′‐GCTAAACACCCAGATGCAAAA‐3′ Reverse 5′‐CGAGGTCTTTTTGGTTTTCC‐3′ |
β‐actin |
Forward 5′‐TGTCACCAACTGGGACGATA‐3′ Reverse 5′‐GGGGTGTTGAAGGTCTCAAA‐3′ |