Skip to main content
. 2021 Oct 20;25(21):10248–10256. doi: 10.1111/jcmm.16963

FIGURE 4.

FIGURE 4

CircGFRA1 acts as a sponge of miR‐1228. (A) The relative levels of circGFRA1, GFRA1, β‐actin (cytoplasmic control) and 18S (nuclear control) were examined. (B) The predicted direct docking sites of miR‐128 within the circGFRA1 sequence. (C) The miR‐1228 content in the HME (184A) cell line, as well asHER‐2‐positive BC cell lines. (D) The relative luciferase activity of BT474 (left) and SKBR3 (right) cells cotransfected with the miR‐1228 mimics and the wild‐type or mutant luciferase enzyme reporter of circGFRA1. (E) MS2‐based RIP assay in HER‐2‐positive BC cell transfects of MS2bs‐circGFRA1, MS2bs‐circGFRA1‐mt or MS2bs‐Rluc