FIGURE 5.

CircGFRA1 silencing enhances ferroptosis in HER‐2‐positive BC. (A) The predicted direct docking sites of miR‐1228 within the AIFM2 sequence. (B) AIFM2 content in the HME (184A) cell line and HER‐2‐positive BC cell lines. (C) The relative luciferase activity of SKBR3 (left) and BT474 (right) cells cotransfected with miR‐1228 mimics, as well as the wild‐type or mutant luciferase reporter of AIFM2. (D) AIFM2 level in cell transfects of miR‐1228 mimics. (E) RIP assay of the relative abundance of circGFRA1, AIFM2 and miR‐1228 in the Ago2 fraction. (F) Cells were transfected, and the relative abundance was determined by an Ago2‐related RIP assay. (G) Western blotting assessment of levels of AIFM2, GPX4 and β‐actin. (H) Quantification and p value of Western Blotting. (I) GSH/GSSG Quantification of the GSH/GSSG ratio