Table 3.
Expression of surface molecules on CD203c+/CD14– basophils in basophilic leukemia patients (#1, #2, #3, #4) and in normal peripheral blood.
| Surface Molecule | CD | #1 | #2 | #3 | #4 | #5 | Normal PB |
|---|---|---|---|---|---|---|---|
| LFA-2 | CD2 | - | - | - | - | n.d. | - |
| Tetraspanin-29 | CD9 | +++ | +++ | ++ | ++ | n.d. | +++ |
| Integrin alpha-M | CD11b | ++ | + | ++ | ++ | ++ | ++ |
| Integrin beta-2 | CD18 | ++ | +/- | ++ | ++ | n.d. | ++ |
| IL-2RA | CD25 | ++ | +/- | + | ++ | n.d. | + |
| DPPIV | CD26 | + | - | ++ | +/- | +/- | + |
| Ki-1 | CD30 | - | - | +/- | +/- | n.d. | - |
| Siglec-3 | CD33 | ++ | + | ++ | ++ | + | ++ |
| Tetraspanin-30 | CD63 | + | + | + | ++ | n.d. | ++ |
| GM-CSFR alpha | CD116 | + | n.d. | + | + | n.d. | + |
| SCFR/KIT | CD117 | + | +/- | + | +/- | - | - |
| IL-3RA | CD123 | +++ | ++ | +++ | +++ | ++ | +++ |
| PD-L1 | CD274 | + | +/- | + | + | +/- | + |
| PD-1 | CD279 | - | - | - | - | n.d. | - |
| Siglec-6 | CD327 | + | +/- | + | + | n.d. | +/- |
| CLL-1 | CD371 | + | + | ++ | ++ | n.d. | ++ |
| FcεRI | n.c. | ++ | n.d. | ++ | ++ | ++ | n.d. |
| IL-1RAP | n.c. | + | +/- | + | +/- | n.d. | - |
The table shows a summary of flow cytometry staining results of basophils obtained from patients #1, #2, #3, #4, and from normal PB. Staining index (SI) for CD203c+ basophils was calculated as ratio of median fluorescence intensities obtained with specific antibodies and isotype-matched control antibodies. Expression (SI) levels were scored as follows: –, SI <1.30; +/–, SI 1.31-3.00; +, SI 3.01-10.00; ++, SI 10.01-100; +++, SI >100.
CD = cluster of differentiation; CLL-1 = C-type lectin-like molecule-1; DPPIV = dipeptidyl peptidase-4; FcecL = high-affinity IgE receptor 1; GM-CSFR alpha = granulocyte-macrophage colony-stimulating factor receptor alpha; IL-1RAP = interleukin-1 receptor accessory protein; IL-2RA = interleukin-2 receptor alpha; IL-3RA = interleukin-3 receptor alpha; LFA-2 = lymphocyte function-associated 2; n.c. = not clustered; n.d. = not determined; PB = peripheral blood; PD-1 = programmed cell death protein 1; PD-L1 = programmed cell death 1 ligand 1; SCFR = stem cell growth factor receptor; Siglec-3 = sialic acid binding immunoglobulin like lectin 3.