FIG. 6.

Cellular osmosensitivity induced by overexpression of a catalytically inactive RCK2(KD) allele. (A) Wild-type (WT) and hog1Δ cells transformed with an empty vector (pCM262) or a plasmid expressing the catalytically inactive RCK2(KD) (pCMkdR2) were grown on YPD or YPD containing NaCl at different concentrations, in the absence of doxycycline (allowing full expression from the Tet promoter). Growth in plates was scored after 3 days at 30°C. (B) Wild-type cells transformed with pCM262 (●) or pCMkdR2 (○) and hog1Δ cells transformed with pCM262 (▾) or pCMkdR2 (▿) were grown in liquid medium in the presence of different concentrations of NaCl, and the effect of stress on cell growth was determined as described in Materials and Methods. Results are means ± standard errors of the means from three independent experiments.