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. 2021 Oct 25;8:715337. doi: 10.3389/fcvm.2021.715337

Figure 5.

Figure 5

RIPK1i suppressed cell apoptosis and necroptosis in plaque. (A–D) Histological analysis of cross-sections from aortic sinus of ApoESA/SA mice. (A) Representative immunofluorescence images of Cleaved Caspase-3 staining (red) in aortic sinus. Scale bar = 500 μm. (B) Quantification of apoptosis (Cleaved Caspase-3 positive) area (n = 5). (C) Representative IHC images of RIP3 in aortic sinus plaque. Scale bar = 100 μm. (D) Quantification of necroptosis (RIP3 positive) area (n = 6). (E) and (F) Peritoneal macrophages isolated from male ApoESA/SA mice were pretreated with RIPK1i (50 ng/ml) or DMSO for 3 h, then stimulated with oxidized LDL (50 μg/ml) for 24 h and stained for TUNEL. (E) Representative immunofluorescence images of TUNEL staining (green). TUNEL positive cells are indicated by the white arrows. Scale bar = 50 μm. (F) Percentage of TUNEL positive cell in peritoneal macrophages (n = 6). All of the data represented as mean ± SEM; *P < 0.05, **P < 0.01. Statistical analysis: unpaired student's t-test (B), non-parametric Mann-Whitney U test (D,F).