KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Cleaved PARP (Asp214) XP rabbit mAb | Cell Signaling Technology | Cat#D64E10 |
| GAPDH mouse mAb | Cell Signaling Technology | Cat#97166 |
| HRP-linked mouse IgG | GE Healthcare | Cat#NA931V |
| HRP-linked rabbit IgG | GE Healthcare | Cat#NA9314V |
| Bacterial and virus strains | ||
| One shot™ Stbl3™ chemical competent E.coli | Thermo Fisher | Cat#C737303 |
| Chemicals, peptides, and recombinant proteins | ||
| BI | (Li et al., 2020) or Lucerna | Cat#600 |
| DFHBI-1T | (Song et al., 2014) or Lucerna | Cat#410 |
| DFHO | (Song et al., 2017) or Lucerna | Cat#500 |
| S-adenosyl-methionine chloride dihydrochloride | Sigma-Aldrich | Cat#A7007 |
| S-adenosyl-methionine iodide | Sigma-Aldrich | Cat#A4377 |
| S-adenosyl-homocysteine | Sigma-Aldrich | Cat#A9384 |
| adenosine | Sigma-Alrdrich | Cat#9251 |
| methionine | Sigma-Aldrich | Cat#M9625 |
| guanine | Sigma-Aldrich | Cat#51030 |
| 5-hydroxytryptophan | Santa Cruz | Cat#278210 |
| cycloleucine | Sigma-Aldrich | Cat#A48105 |
| TRIzol™ LS Reagent | Invitrogen | Cat#10296010 |
| doxorubicin | Sigma-Aldrich | Cat#D1515 |
| Halt protease and phosphatase inhibitor cocktail | ThermoFisher | Cat#78440 |
| Pierce ECL Substrate | ThermoFisher | Cat#32106 |
| Critical commercial assays | ||
| AmpliScribe™ T7-Flash™ transcription kit | Lucigen | Cat#ASF3507 |
| Micro Bio-Spin Columns with Bio-Gel P-30 | Bio-Rad | Cat#7326223 |
| FuGENE HD | Promega | Cat#2311 |
| Bridge-It® SAM Fluorescence Assay Kit | Mediomics | Cat#1-1-1003 |
| Pierce BCA Protein Assay Kit | ThermoFisher | Cat#23225 |
| Deposited Data | ||
| Bacteriophage Phi-29 RNA structure | (Zhang et al., 2013) | PDB: 4KZ2 |
| Phage Twort group 1 ribozyme structure | (Golden et al., 2005) | PDB: 1Y0Q |
| Haloarcula marismortui 23S ribosomal RNA structure | (Klein et al., 2004) | PDB: 1S72 |
| Bacillus subtillis ribonuclease P B-type structure | (Krasilnikov et al., 2003) | PDB: 1NBS |
| Experimental models: cell lines | ||
| HEK293T/17 | ATCC | Cat#CRL-11268 |
| HeLa | ATCC | Cat#CCL-2 |
| Oligonucleotides | ||
| RiboRuler Low Range RNA Ladder | ThermoFisher | Cat#SM1831 |
| Table S1 | This paper | N/A |
| Recombinant DNA | ||
| pAV-Tornado-Broccoli-SAM Sensor | (Litke and Jaffrey, 2019) | N/A |
| pAV-Tornado-F30-Broccoli-SAM sensor | This paper | N/A |
| pAV-Tornado-Broccoli-SAM Sensor Mutant | This paper | N/A |
| pAV-Tornado-Broccoli-Stable Control | This paper | N/A |
| Software and algorithms | ||
| Fiji | NIH | fiji.sc |
| NIS-Element | Nikon | www.nikon.com |
| Prism 8 | GraphPad | www.graphpad.com |
| PyMol | Schrödinger | pymol.org |