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Effect of mismatches between probes and template on PCR reaction. (A) Ct values detected when the US N1 probe contained mismatch with the template. (B) Ct values detected when the US N2 probe contained mismatch with the template. (C) Ct values detected when the US N3 probe contained mismatch with the template. Student’s t-test by SPSS 15 was used for data analysis. A p-value < 0.05 was considered statistically significant. *p < 0.05; **p < 0.01.
