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Point mutations affect the thermal stability of the corresponding domains. (A) Thermal transition midpoint temperature difference of the mutant domains at positions W108 and W132. (B) Thermal transition midpoint temperature difference of the mutant domains at positions W301 and M322. Student’s t-test was used to conduct statistical analysis, and differences were considered significant when p <0.05, **p < 0.01, and ***p < 0.001.
