. 2021 Oct 25;8:760578. doi: 10.3389/fnut.2021.760578

Table 2.

Melatonin concentration of in vitro fermentation (pg/mL).

Item	Treatment			SEM	Time	Treatment	Ti × Tr
	CK0	CK1	MLT
0 h	48.17^b	50.39^b	99.83^a		^***	^***	^**
4 h	47.90^b	46.48^b	84.64^a
8 h	34.19^b	41.82^b	82.38^a
12 h	46.40^b	42.58^b	87.01^a	12.899
16 h	29.54^b	28.72^b	79.42^a
20 h	31.88^b	24.91^b	52.96^a
24 h	28.08^ab	15.79^b	57.82^a
Decrease rate (pg·mL⁻¹·h⁻¹)	0.84^b	1.44^a	1.75^a

In the same row, values with no letter or the same letter superscripts mean no significant difference (P > 0.05), whereas with different superscripts mean significant difference (P < 0.05). CK0, non-rumen fluid + non-melatonin; CK1, rumen fluid + non-melatonin; MLT, rumen fluid + melatonin.

^**

P < 0.01, and

^***

P < 0.001.