Table 3.
Physicochemical Characteristics of the CA-Loaded NS Formulations and Marketed Product (Madeca Cream)
| 1%NS | 5%NS | 10%NS-1 | 10%NS-2 | Madeca Creamb | |
|---|---|---|---|---|---|
| Compositions | |||||
| CA (mg) | 10.0 | 50.0 | 100.0 | 100.0 | |
| PVP K30 (mg) | 5.0 | 5.0 | 5.0 | 5.0 | |
| BG (mL) | - | - | - | 0.5 | |
| DW (mL) | 1.0 | 1.0 | 1.0 | 0.5 | |
| Physicochemical characteristics | |||||
| AS content (mg/mL)a | 3.49 ± 0.03 | 17.19 ± 0.48 | 33.75 ± 0.12 | 29.90 ± 0.66 | 2.77 ± 0.04 |
| MA content (mg/mL)a | 3.25 ± 0.02 | 15.44 ± 0.67 | 30.37 ± 1.16 | 26.90 ± 0.04 | 3.03 ± 0.20 |
| AA content (mg/mL)a | 2.49 ± 0.01 | 10.23 ± 0.32 | 18.79 ± 0.74 | 15.43 ± 0.08 | 1.72 ± 0.21 |
| Particle size (nm)a | 493.5 ± 0.8 | 268.4 ± 2.1 | 201.4 ± 5.0 | 379.4 ± 6.3 | –c |
| Homogeneity (PDI)a | 0.17 ± 0.01 | 0.24 ± 0.00 | 0.24 ± 0.01 | 0.20 ± 0.30 | –c |
| pHa | 5.72 ± 0.07 | 5.40 ± 0.01 | 5.32 ± 0.05 | 5.65 ± 0.01 | –c |
Notes: aData are expressed as mean ± SD (n=3); bThe exact composition of Madeca cream is unknown, and only the content of AS, MA, and AA contained in cream formula was evaluated; cNot determined.
Abbreviations: CA, Centella asiatica; BG, butylene glycol; NS, nanocrystal suspensions; AS, asiaticoside; AA, asiatic acid; MA, madecassic acid.