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Indirect cytocompatibility evaluation. (ion release approach). Samples were incubated in DMEM (37 °C, 120 rpm) for 1day and 5days, and the supernatant was used to cultivate hMSC in them A) Relative fluorescence unit (RFU) of BMSC B) Fluorescent staining by Live/Dead Viability/Cytotoxicity Kit after 5days, C) detection of ions inside the cells' cytoplasm with staining with May Grunwald and Giemsa after 5days.
