Figure 2.

Characteristics of CD4⁺ T cell repertoire in the bilateral tumor. (A), V/J segment usage plots of the bilateral CD4⁺ T cell repertoires. Ribbons connecting the V and J segments are scaled by the corresponding V/J pair frequency. The color of the circumference represents the same V and J genes, and the color of the ribbon represents the same J genes (n = 4). (B), Principal component analysis of V and J segment usage of tumor-infiltrating CD4⁺ T cells. Dots of the same color indicate left and right tumors of the same individual (n = 4). (C), Frequency of abundant CD4⁺ T cell clones in the tumor and dLN. CD4⁺ T cell clones were categorized into five classes based on their rank in each repertoire: top 10, 11^th–20^th, 21^st–40^th, 41^st–100^th, and others. The total frequency of clones in each class is shown (n = 4). (D), Clonality of the left and right CD4⁺ tumor repertoire (n = 4). (E), Homoscedasticity plot for variance of clonality in individual mice. The X-axis represents the average clonality of bilateral tumors in each individual (n = 4). The Y-axis represents variance of clonality between different bilateral tumors within individuals. Sum of squares within mouse = 4.4 × 10^-4, Sum of squares between mice = 1.3 × 10^-2, Degree of freedom within mouse = 4, Degree of freedom between mice = 3.