Fig. 2.

Overexpression of DU promotes SeV/VSV proliferation. (A) 293T cells were transfected with pFlag-CMV-DU expression plasmid or empty vector, 12 h after transfection, cells were infected with 0.5 MOI SeV or VSV. The mRNA level of SeV or VSV was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis at 16 h post-infection. (B and C) 293T cells were transfected with different doses of pFlag-CMV-DU expression plasmids (100 ng/300 ng/500 ng), 12 h after transfection, cells were infected with SeV or VSV and the mRNA level of SeV or VSV was tested by RT-qPCR analysis at 16 h post-infection. (D and E) 293T cells were transfected with pFlag-CMV-DU expression plasmid or control plasmid, after 12 h, cells were infected with VSV-GFP, immunofluorescence microscope imaging (D), and Western blot (E) used for observing viral proliferation at 16 h post-infection. Molecular weight markers in kDa are shown on the right. Results were normalized to those of the control gene β-actin and are presented relative to those of control cells. Differences in data considered statistically significant at p-value less than 0.05 (^* P<0.05, ^** P<0.01, ^*** P<0.001, ^**** P<0.0001, and NS: No significant). CMV: Cytomegalovirus, DU: dUTPase, MOI: Multiplicity of infection, SeV: Sendai virus, and VSV-GFP: Vesicular stomatitis virus harbored green fluorescence protein gene