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Table 1.

Primers used for PCR amplification

Primer name GenBank No. Sequence of primer (5´-3´)
pcDNA3.1-DU-F GU120138.1 CCACACTGGACTAGTGGATCCATGGCAGGATATGATTTAATATGT
pcDNA3.1-DU-R CTTGGTACCGAGCTCGGATCCTAATATTAATTGTGCAAACTTT
Flag-CMV2-DU-F GU120138.1 CGGAATTCAATGGCAGGATATGATTTAATATGT
Flag-CMV2-DU-R GCTCTAGATCATAATATTAATTGTGCAAACTTT

PCR: Polymerase chain reaction, and CMV2-DU: CMV promotor DU expression plasmid